Eco-Friendly Applications of Bacterial Extracellular Alkaline Protease

Optimization of Extracellular Alkaline Protease Enzyme from Bacillus Sp

Proteases are very important industrial enzymes, which contribute about 60% of the total world enzyme market. Among the various proteases, bacterial proteases are the most significant, compared with animal and fungal proteases (Rahman et al., 1994) and among bacteria, Bacillus sp are specific producers of extra-cellular proteases (Coolbear et al., 1992; Banerjee et al., 1999). These enzymes hav...

Cloning and Enhanced Expression of an Extracellular Alkaline Protease from a Soil Isolate of Bacillus clausii in Bacillus subtilis

in the detergent industry. In this study, the extracellular alkaline serine protease gene, aprE, from Bacillusclausii was amplified by PCR and further cloned and expressed in B. subtilis WB600 using the pWB980 expression vector. Protease activity of the recombinant B. subtilis WB600 harboring the plasmid pWB980/aprEreached up to 1020 U/ml, approximately 3-folds higher than the nativ...

Eco-friendly Dentistry

Enhanced Thermo-stability of Bacterial Alkaline protease by calcium ions

The purpose of this study is to improve the thermo-stabilty of alkaline protease produced by Bacillus sp. using rice bran during solid state fermentation. After partial purification of alkaline protease, various parameters namely effect of temperature and metal ions on enzyme activity was investigated. The suitable temperature for alkaline protease activity was observed at 50°C. Among various m...

Recommending Eco-Friendly Route Plans

As personal transportation is one of the greatest contributors of CO2 emissions, means able to assist travelers in reducing their ecological impact are urgently needed. In this work we focus on travel recommenders that encourage green transportation habits among travelers who have a preexisting interest in taking action to lessen their impact on the environment. We aim to provide urban traveler...